Check that all bottles are filled with the appropriate solutions. Please read the user manual of this instrument carefully and pay attention to all warnings and precautions. Keep this user manual in a safe place, accessible for anyone using the. After priming, the instrument will automatically display the status menu. If you make the solutions they should be filtered before use use a 0. Jul 18, 2016 automacs pro separator magnetic cell sorter available from the lab world group. If you want to use it inside the hood, do not forget to. Maintenance and troubleshooting for magnetic separator. Easy to use automated magnetic cell sorting biocompare. Alfa laval separators manuals and spare parts catalogs in pdf. Uantitative analysis of 3color fluorescent western blots with the fluorchem q. When using the program posselwb, the whole blood sample will be diluted 3fold the starting volume. Switch on the automacs pro separator the instrument will automatically initialize.
The macs minisampler connects to the automacs pro separator and thus represents a part of the cell separation device. Indicates a hazard situation, which if not avoided, could result in minor or moderate injury. The automacs pro separator is equipped with reusable automacs columns. Unless otherwise specifically indicated, miltenyi biotec products and services are for research use only and not for therapeutic or diagnostic use. The following warnings and cautions are provided to help you prevent injury to yourself or damage to the instrument. Persons who have not been briefed on proper and correct operation. Warnings and precautions the guidelines in this section explain the potential risks associated with the. Alfa laval separators manuals and spare parts catalogs in. Consult the user manual before operating this instrument for the first time.
Alfa laval p 605 separator operation and service instruction 401240 pa 625 alfa laval pa625 separator final drawings 401250 pu 100 alfa laval purifier unit pu100 separator full set manuals. Columns do not have a top or bottom and do not require special orientation in the column holder. Program choice depends on the isolation strategy, the strength of magnetic labeling, and the frequency of magnetically labeled cells. Designed for the selection of target cells in a fully automated, walkaway fashion. For manual labeling follow labeling instructions in the macs microbeads datasheet. Keep this user manual in a safe place, accessible for anyone using the automacs pro separator. It is a computercontrolled device for the automated separation of magnetically labeled cells using macs technology. We are a largescale manufacturer specializing in producing various mining machines including different types of sand and gravel equipment, milling equipment, mineral processing equipment and building materials equipment. This chapter describes the safety instructions and site requirements for your automacs pro separator.
Automacs pro separator auto pro provided by the beijing biolaunching technologies co. Persons who have not read and understood these basic safety precautions. Mif inhibition reverts the gene expression profile of human melanoma cell lineinduced mdscs to normal monocytes. Apr 24, 2012 overview the automacs pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple samples. Depletion with the automacs pro separator or the automacs separator refer to the respective user manual for instructions on how to use the automacs pro separator or the automacs separator. Column substitutes for longterm storage are provided with the automacs pro separator starter kit. Miltenyi biotec is a global provider of products and services that advance biomedical research and cellular therapy. Automacs user guide the automacs allows biomagnetic cell sorting of cells labelled with macs microbeads. Automacs pro separator magnetic cell sorter youtube. Protocols section of the respective separator user manual. Here, we have developed a methodology for bacterial cell sorting using the semiautomated automacs pro separator for the first time, and have produced a complete method for sorting of bacteria.
The automacs pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple samples. Cell sorters core facility flow cytometry bmc lmu munich. The epfl flow cytometry core facility fccf provides stateoftheart instrumentation, technical expertise, theoretical and technical training, and cell sorting service. If in doubt contact your local sales representative or miltenyi biotec automacs pro specialist. This chapter describes the safety instructions and site. The automacs pro separator employs stateoftheart technology. Content 5 content 5 content chapter 1 15 1 important information 15 1. Rinsing buffer pbs, 2mm edta pbs, 2mm edta or facsrinse.
Mif inhibition reverts the gene expression profile of. The macs products are sold by miltenyi biotec online catalogue at. Morrison 9102014 page 23 the automacs pro separator is a benchtop instrument for highspeed automated magnetic cell sorting of multiple samples under standardized conditions. Once installed, the automacs columns become part of a closed fluidic system and can be used for up to 14 days or 100 separations within 14 days. If the separator has been delivered and installed by alfa laval as a part of a processing system, this manual should be. For details refer to the section describing the cell separation programs in the respective user manual. Automacs pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple sam.
A utomacs pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple samples. The automacs pro separator is a benchtop automated magnetic cell sorter for the processing of multiple samples. Automated cell isolation with the automacs pro separator provides fast and gentle isolation of virtually any cell type. Magnetic separation with the automacs pro separator or the automacs separator refer to the respective user manual for instructions on how to use the automacs pro separator or the automacs separator. Employing the macs technology, the automacs separator is designed for positive selection as well as depletion of magnetically labeled cells. Buffers used for operating the automacs pro separator or the automacs separator should have a temperature of.
After each cell separation, a thorough washing procedure rinses the columns of the automacs pro separator. Aug 21, 2018 the automacs pro separator is a benchtop magnetic cell sorter that allows gentle sorting of more than ten million cells per second from a sample of up to 4. Maximal care has been taken by miltenyi biotec in the preparation of this user manual. Operating instructions, service manual, spare parts catalogue, system description, parameter list, alarms and fault finding, system reference installation. Empty the waste bottle and add 100ml bleach to the bottom. To view the current software version, from the software menu select. Stanford automacs pro user guide 072211 starting up 1.
The automacs pro separator is designed for safe use when installed correctly, operated by trained personel operated in accordance with general safety practices and the instructions in this user manual. Save time with fully automated multisample cell separation. If the separator has been delivered and installed by alfa laval as. Method for discovery of peptide reagents using a commercial. Persons who have not read and understood the instruction manual of the separator. Automated cell isolation automacs pro separator usa. Manual and the installation manual should be interpreted so as. The macs minisampler, attached to the automacs pro separator, can hold one of three different macs cooling tube racks containing up to six samples.
Automacs pro separator magnetic cell sorter available from the lab world group. Identification of comigrating proteins on the fluorchem m system. The cell separation is programmed using the touchscreen and intuitive screen menus. Employing macs technology, the automacs pro separator is designed for cell isolation in a fully automated, walkaway fashion.
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